Open Access Research article

Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response

Valentina Coppola1, Mariangela Coppola1, Mariapina Rocco2, Maria Cristina Digilio1, Chiara D’Ambrosio3, Giovanni Renzone3, Rosanna Martinelli4, Andrea Scaloni3, Francesco Pennacchio1, Rosa Rao1* and Giandomenico Corrado1

Author Affiliations

1 Dipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, NA, Italy

2 Università degli Studi del Sannio, 82100 Benevento, Italy

3 Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo-CNR, 80147 Napoli, Italy

4 CEINGE, 80145 Napoli, Italy

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BMC Genomics 2013, 14:515  doi:10.1186/1471-2164-14-515

Published: 29 July 2013

Additional files

Additional file 1: Table S1.:

Primers used for the expression study and their main features. Table S2: Differentially expressed genes 24 h after infestation with M. euporbiae. Table S3: Differentially expressed genes 48 h after infestation with M. euphorbiae. Table S4: Differentially expressed genes 96 h after infestation with M. euphorbiae. Table S5. Protein species showing quantitative changes during aphid infestation as identified by combined 2-DE and MS procedures.

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Additional file 2: Figure S1.:

Microarray validation and concordance with Real Time results (A) Relative gene expression analysis 24 h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-∆Ct values were significantly different from the calibrator (p < 0.01; Student’s t-test). (B) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale. Figure S2: Microarray validation and concordance with Real Time results (C) Relative gene expression analysis 48 h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-∆Ct values were significantly different from the calibrator (p < 0.01; Student’s t-test). (D) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale. Figure S3: Microarray validation and concordance with Real Time results (E) Relative gene expression analysis 96 h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-∆Ct values were significantly different from the calibrator (p < 0.01; Student’s t-test). (F) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale.

Format: PPTX Size: 130KB Download file

Open Data