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Open Access Research article

Genome-wide identification, molecular cloning, expression profiling and posttranscriptional regulation analysis of the Argonaute gene family in Salvia miltiorrhiza, an emerging model medicinal plant

Fenjuan Shao and Shanfa Lu*

Author Affiliations

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No.151, Malianwa North Road, Haidian District, Beijing 100193, China

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BMC Genomics 2013, 14:512  doi:10.1186/1471-2164-14-512

Published: 29 July 2013

Additional files

Additional file 1:

Conserved domains in SmAGO proteins. DUF1785, PAZ and PIWI domains are shown.

Format: PDF Size: 31KB Download file

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Open Data

Additional file 2:

Alignment of the deduced SmAGO amino acid sequences. DUF1785, PAZ and PIWI domains are indicated by dotted, solid and broken lines. The conserved DDH/H798 or DDD/H798 residues are indicated by arrows.

Format: PDF Size: 499KB Download file

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Open Data

Additional file 3:

Primers used for 5′-RACE of SmAGOs. Complete set of primers used for 5′-RACE of SmAGOs.

Format: PDF Size: 25KB Download file

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Open Data

Additional file 4:

Primers used for 3′-RACE of SmAGOs. Complete set of primers used for 3′-RACE of SmAGOs.

Format: PDF Size: 25KB Download file

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Open Data

Additional file 5:

Primers used for amplification of full-length SmAGOs. Complete set of primers used for amplification of full-length SmAGOs.

Format: PDF Size: 25KB Download file

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Open Data

Additional file 6:

Primers used for qRT-PCR. Complete set of primers used for qRT-PCR.

Format: PDF Size: 8KB Download file

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Open Data

Additional file 7:

Primers used for miRNA quantification. Complete set of primers used for miRNA quantification.

Format: PDF Size: 7KB Download file

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Open Data

Additional file 8:

Primers used for mapping of SmAGO cleavage sites. Complete set of primers used for mapping of SmAGO cleavage sites.

Format: PDF Size: 8KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data