Additional file 2: Figure S1.

Ditag coverage by chromosomes. A) Restriction sites covered by the experimental ditags corresponding to the reference; B) Genomic regions covered by the restriction fragments tagged by the experimental ditags. Figure S2. Venn diagram of A) the number of Ref-Ditags covered by the two libraries; B) the deletions identified using ditags from Lib1, Lib2 and the combined data. Figure S3. A 3075-bp heterozygous deletion that skips 5 consecutive restriction sites on chromosome 17. Ditags were used to design a pair of primers to amplify the breakpoint-containing sequences. The results showed two bands representing the reference and mutant bands, respectively. The breakpoint sequence was identified by direct Sanger sequencing. Figure S4. Flow-chart of the ditag library construction process. Figure S5. Nick-translation distances of the two libraries inferred from the reads alignment.

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Gong et al. BMC Genomics 2013 14:51   doi:10.1186/1471-2164-14-51