A 3290-bp deletion that skips three consecutive restriction sites on chromosome 17. Ditags were used to design a pair of primers to amplify the breakpoint-containing sequences. The results showed bands of different sizes for the control and the test DNA. The breakpoint sequence was identified by direct Sanger sequencing with a micro-insertion observed at the break sites.
Gong et al. BMC Genomics 2013 14:51 doi:10.1186/1471-2164-14-51