Ditag insert size analysis. A) The size distribution of restriction fragments were inferred from the ditags based on the reference sequence. The asterisks indicate the significance (p<0.001) according to a t-test. B) Distribution of ditag coverage as a function of restriction fragment size. The size distribution of restriction fragments that were generated from the hg18 reference sequence is indicated with a green line, with the vertical dashed lines indicating the average and median sizes. The observed ditag frequencies as a function of fragment size are indicated by the blue and cyan lines (one for each library). The grey line shows the average ditag coverage distribution over various fragment sizes. The horizontal dashed lines show the average and median frequency values.
Gong et al. BMC Genomics 2013 14:51 doi:10.1186/1471-2164-14-51