Additional file 3: Figure S3.

Phylogenetic tree of DBM and Bombyx mori ABC transporter genes. The amino acid sequences (60 aa or longer) of nucleotide-binding domains (NBDs) (Pfam ID: PF00005) were extracted (57 NBDs from 54 ABC transporter genes in DBM; 70 NBDs from 52 ABC transporter genes in B. mori) and aligned using ClustalW. To avoid “No common sites” error in MEGA5, 6 NBDs (3 genes) of B. mori were removed, resulting in 64 NBDs from 49 ABC transporter genes. Phylogenetic tree was built using neighbor-joining method in MEGA5 with a bootstrap of 1,000 replicates, Poisson model for amino acid substitution, and pairwise deletion of gaps. Bootstrap values resulting from 1,000 replicates are shown at the branch points. The number (1 or 2) in parentheses is added to ID of each ABC transporter gene containing two NBDs. Each DBM gene ID (PxABC-XXXXXX) corresponds to PXGS_V2_XXXXXX in KONAGAbase.

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Jouraku et al. BMC Genomics 2013 14:464   doi:10.1186/1471-2164-14-464