Additional file 2: Figure S2.
Phylogenetic tree of DBM and Bombyx mori GST genes. The amino acid sequences (38 aa or longer) of Glutathione S-transferase, N-terminal (GST_N) domain (Pfam ID: PF02798) were extracted (20 domains in DBM; 23 domains in B. mori) and aligned using ClustalW. Phylogenetic tree was built using neighbor-joining method in MEGA5 with a bootstrap of 1,000 replicates, Poisson model for amino acid substitution, and pairwise deletion of gaps. Bootstrap values resulting from 1,000 replicates are shown at the branch points. Each DBM gene ID (PxGST-XXXXXX) corresponds to PXGS_V2_XXXXXX in KONAGAbase.
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Jouraku et al. BMC Genomics 2013 14:464 doi:10.1186/1471-2164-14-464