Verification of selected genes from the microarray data by RTqPCR. The six mitochondrial genes that were expressed at 400-fold or greater in the AR-5 line as compared to the IVB line, were selected for verification. The ΔΔCT method was used for quantitation and the data was normalized to 60S ribosomal protein L27a (RpL27a). All p-values are ≤ 0.0005. The genes verified by RTqPCR were (MT-CO1) Cytochrome c oxidase subunit 1, (MT-CO2) Cytochrome c oxidase subunit 2, (MT-CYB) Cytochrome b, (MT-ND1) NADH ubiquinone oxidoreductase chain 1, (MT-ND2) NADH ubiquinone oxidoreductase chain 2, and (MT-ND3) NADH ubiquinone oxidoreductase chain 3.
Dalwadi and Uht BMC Genomics 2013 14:413 doi:10.1186/1471-2164-14-413