Archaeal and eukaryotic nucleosomes are positioned by conserved sequences. The frequencies of occurrence of AA/AT/TA/TT and CC/GG/GC/CG dinucleotides, at each position relative to the center of (a) eukaryotic nucleosomes assembled by chicken histones on T. kodakarensis genomic DNA, and (b) of archaeal nucleosomes assembled by T. kodakarensis histones on yeast genomic DNA. (c) The ratio of the presence and absence of all pentamers in the T. kodakarensis DNA protected from MN digestion by assembly into eukaryotic nucleosomes, and (d) in yeast DNA assembled into archaeal nucleosomes. As indicated, all pentamers that contained only G and/or C were preferentially incorporated into both eukaryotic and archaeal nucleosomes (blue regions) whereas all pentamers that contained only A and/or T (red region) were preferentially excluded nucleosomes.
Nalabothula et al. BMC Genomics 2013 14:391 doi:10.1186/1471-2164-14-391