Open Access Research article

Archaeal nucleosome positioning in vivo and in vitro is directed by primary sequence motifs

Narasimharao Nalabothula1, Liqun Xi2, Sucharita Bhattacharyya3, Jonathan Widom3, Ji-Ping Wang2, John N Reeve4, Thomas J Santangelo45 and Yvonne N Fondufe-Mittendorf1*

Author Affiliations

1 Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA

2 Department of Statistics, Northwestern University, Chicago, IL 60208, USA

3 Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA

4 Department of Microbiology, Ohio State University, Columbus, OH 43210, USA

5 Center for RNA Biology, Ohio State University, Columbus, OH 43210, USA

For all author emails, please log on.

BMC Genomics 2013, 14:391  doi:10.1186/1471-2164-14-391

Published: 10 June 2013

Additional files

Additional file 1: Figure S1:

Shows data that document that archaeal nucleosomes assembled by HTkA and HTkB, in vivo and in vitro, contain 5 bp offset helical repeats of AA/AT/TA/TT and CC/GG/GC/CG dinucleotides and preferentially exclude oligo A/T-rich sequences.

Format: PDF Size: 202KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2: Figure S2:

Documents the conserved positioning of archaeal and eukaryotic histone assembly into nucleosomes on Methanothermobacter thermautotrophicus genomic DNA.

Format: PDF Size: 43KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3: Figure S3:

Documents the absence of archaeal nucleosome assembly in vivo and in vitro on the two rDNA operons present in the Methanothermobacter thermautotrophicus genome.

Format: PDF Size: 48KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data