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Open Access Highly Accessed Research article

Pyrosequencing analysis of the human microbiota of healthy Chinese undergraduates

Zongxin Ling1, Xia Liu2, Yueqiu Luo1, Li Yuan1, Karen E Nelson3, Yuezhu Wang4, Charlie Xiang13* and Lanjuan Li1*

Author Affiliations

1 State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China

2 Department of Intensive Care Unit, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China

3 J. Craig Venter Institute, Rockville, Maryland, 20850, USA

4 Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China

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BMC Genomics 2013, 14:390  doi:10.1186/1471-2164-14-390

Published: 10 June 2013

Additional files

Additional file 1: Table S2:

Comparison of phylotypes coverage and diversity estimation of the 16S rRNA gene libraries for individuals at 3% dissimilarity from the pyrosequencing analysis.

Format: DOC Size: 97KB Download file

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Additional file 2: Figure S1:

Rarefaction curves were used to estimate richness (in this case the number of taxa at a 3% dissimilarity level) among individuals. The vertical axis shows the number of OTUs that would be expected to be found after sampling the number of tags or sequences shown on the horizontal axis.

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Additional file 3: Table S1:

All genera assigned using the RDP-classifier with at least 50% as bootstrap support).

Format: XLS Size: 349KB Download file

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