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Time-dependent changes in gene expression induced by secreted amyloid precursor protein-alpha in the rat hippocampus

Margaret M Ryan123*, Gary P Morris135, Bruce G Mockett14, Katie Bourne13, Wickliffe C Abraham14, Warren P Tate13 and Joanna M Williams12

Author Affiliations

1 Brain Health Research Centre, University of Otago, PO Box 56, Dunedin New Zealand

2 Department of Anatomy, Otago School of Medical Sciences, PO Box 913, Dunedin New Zealand

3 Department of Biochemistry, Otago School of Medical Sciences, PO Box 56, Dunedin New Zealand

4 Department of Psychology, University of Otago, PO Box 56, Dunedin New Zealand

5 Neuroscience Department, Garvan Institute of Medical Research, Sydney, Australia

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BMC Genomics 2013, 14:376  doi:10.1186/1471-2164-14-376

Published: 6 June 2013

Additional files

Additional file 1: Figure S1:

Neuroprotective effects of sAPPα are concentration dependent. Change in cell death in DG/ob following treatment of sAPPα (0.03-10 nM) relative to control/NMDA treatment. *:p < 0.05, n = 4 animals, 19 slices/group. sAPPα neuroprotection followed a U-shaped dose–response curve, with increasing concentrations of sAPPα (to a maximum of 1 nM) leading to a significant neuroprotective effect, which then decreased with increasing concentrations of sAPPα. This agrees with our previous study [16], where we observed a dose dependent facilitation of in vivo LTP by sAPPα, with little effects on LTP at low concentrations, facilitation at mid-range concentrations and significant inhibition at high concentrations. This may reflect the occupancy/binding properties of the receptor of sAPPα, as yet unidentified [12,97]. No significant effects were detected following incubation with the other sAPPα + NMDA concentrations.

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Additional file 2: Tables S1-S4:

Significantly expressed transcripts clusters following 15 min (Table S1), 2 h (Table S2) and 24 h (Table S3) sAPPα exposure. Table S4: Validation of selected differentially expressed genes by qPCR including associated p-values. Entries without gene symbols and titles have no annotations associated with these probe ids.

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Additional file 3: Table S5:

Expression of sAPPα related genes across time. Overlapping temporal gene expression lists from Venn diagram (Figure 2B). NC: no change in gene expression at this time.

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Additional file 4: Table S6:

DAVID functional annotation clustering of sAPPα-regulated genes.

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Additional file 5: Figure S2:

Genes with annotations related to neurogenesis and development. Associations between the 24 h gene list and their annotations following DAVID functional analysis. The region in green illustrates that all annotations are common across the cluster. Enrichment score 2.35.

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Additional file 6: Table S7:

List of primers used for real-time qPCR.

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