Condition-specific LNS sorting of expression data into conserved and non-conserved patterns. A. Expression data from the diauxic shift in both S. bayanus and S. cerevisiae were separately zero-transformed, and then aligned so that each horizontal line of data contains the expression of an orthologous pair of genes. The paired expression data were hierarchically clustered by uncentered Pearson correlation . The data are presented by a heat map of log2 expression values. S. cerevisiae diauxic shift data were from . B. The clustered data were partitioned by LNS score into quartiles, preserving the order of the genes in the original cluster. Genes with different expression between the species have low LNS scores. C. Cell cycle data from MATa cells synchronized by alpha factor arrest were mean centered. S. cerevisiae cell cycle data was obtained from . For each species, the phase of the cell cycle was determined by Fourier transformation, and the top genes mapped to the phase were determined as cell cycle-regulated. Orthologs with either member of the ortholog pair determined as cell cycle-regulated are presented. The orthologs were arranged by the time of peak expression in S. bayanus.
Guan et al. BMC Genomics 2013 14:33 doi:10.1186/1471-2164-14-33