Expression pattern validation of selected genes by qPCR. Changes in transcript levels of 30 selected genes as detected by qPCR. A, B, and C represent 10 up-regulated, 10 down-regulated, and 10 unigenes with changes in transcript abundance of < 2-fold, respectively. X-axis shows -fold change in transcript abundance of unigenes. Black bar indicates transcript abundance changes calculated by the RPKM method. Red bar with associated standard error bar represents relative expression level determined by qPCR using 2-∆∆CT method. Results represent mean standard deviations (± SD) of three experimental replicates.
Dang et al. BMC Genomics 2013 14:29 doi:10.1186/1471-2164-14-29