Table 2

Allelic composition of the parents and progeny of the F2 hybrid backcross
Progeny sample G Fam Petal colour Ke923 (MYB110a) alleles Ke701 (MYB110b) alleles
1 2 1 2 3 4 5 6 7 8 9 10
21-05-08g m 1 red 209 228 361 426
21-05-08j m 1 red 209 228 361 426
21-07-09c f 1 red 209 228 361 426
21-06-10h f 1 red 209 228 361 427
21-07-09f m 1 red 209 228 361 427
21-07-09g f 1 red 209 228 361 427
21-07-09b f 1 white 228 410 426
21-07-12e m 1 white 228 410 426
21-08-08h f 1 white 228 410 426
21-07-09a m 1 white 228 410 427
21-07-12b f 1 white 228 410 427
21-08-08b f 1 white 228 410 427
21-05-09f m 2 red 209 228 359 426
21-07-13d m 2 red 209 228 359 426
21-06-11i f 2 red 209 228 359 427
21-07-13e f 2 red 209 228 359 427
21-08-11a m 2 red 209 228 359 427
21-05-09b m 2 white 228 426 432
21-05-09e f 2 white 228 426 432
21-05-09h m 2 white 228 426 432
21-05-09g f 2 white 228 427 432
21-07-13g f 2 white 228 427 432
21-07-14g m 2 white 228 427 432
21-05-12i f 3 red 209 228 359 426
21-07-11c m 3 red 209 228 359 426
21-09-10c f 3 red 209 228 359 426
21-05-12a f 3 red 209 228 359 427
21-05-12g f 3 red 209 228 359 427
21-07-11b m 3 red 209 228 359 427
21-06-13a m 3 white 228 426
21-08-09h f 3 white 228 427
21-06-08a f 4 red 209 228 359 426
21-06-12b m 4 red 209 228 359 426
21-07-08h f 4 red 209 228 359 426
21-07-10b m 4 red 209 228 359 426
21-09-11f m 4 red 209 228 359 426
21-06-08h f 4 red 209 228 359 427
21-07-10h f 4 red 209 228 359 427
21-09-09a f 4 red 209 228 359 427
21-09-09d m 4 red 209 228 359 427
21-07-08a m 4 white 228 426
21-07-08b f 4 white 228 426
21-07-10e f 4 white 228 426
21-07-10f m 4 white 228 426
Parents
CK01_03 f white nd 410 413
CK01F2 f white 228 406 408
CK15_01 m white 228 426
CK15_02 m white 228 426 427
CK21_01 f white 228 409 432
EA03_01 f red 209 359 361
EA04_03 f red 209 228 359
EA07_R22 m red 209 228 nd
Hort16A f white 228 406 426
11-06-17c f 1 red 209 228 361 410
11-06-16e f 2 red 209 228 359 432
11-06-15d f 3 red 209 228 359 426
11-06-15c f 4 red 209 228 359 426

Analysis by capillary electrophoresis of PCR products with the MYB110 gene markers showed that both MYB110a and MYB110b carried alleles that segregated with red petal colour. In all the parents available for analysis, and progeny, only two alleles were amplified with MYB110a marker primers. Red-petalled Actinidia genotypes carried alleles represented by PCR products sized 209 (allele 209) and 228 (allele 228), while white-petalled genotypes were homozygous for allele 228. Analysis with MYB110b showed a total of ten alleles amplified in parents. Of these ten alleles, only six were found in the F2 backcross progeny. Two of the six alleles, alleles 359 and 361, segregated with red petal colour. The presence of either allele gave the red phenotype.

Fraser et al.

Fraser et al. BMC Genomics 2013 14:28   doi:10.1186/1471-2164-14-28

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