Gel electrophoresis banding patterns identify red-petal progeny. Progeny of four interspecific hybrid families between (Actinidia chinensis var. chinensis x Actinidia eriantha) x Actinidia chinensis var. chinensis had either red or white-petalled flowers. Gel electrophoresis of the PCR products amplified with primers to the MYB110 genes showed that the genotypes could be distinguished on the basis of their banding patterns. With MYB110a, (primer pair Ke923) genotypes with red petal colour all had two clear bands, while genotypes with white petal colour had a single band (Figure 6 a). Primer pair Ke701, specific to MYB110b, also segregated with red petal colour (Figure 6 b). In this instance the red petal phenotype had three bands while the white petal genotype had one band. Both gels were run through a 2.75% agarose gel with a 1Kb + size ladder.
Fraser et al. BMC Genomics 2013 14:28 doi:10.1186/1471-2164-14-28