Open Access Highly Accessed Research article

An R2R3 MYB transcription factor determines red petal colour in an Actinidia (kiwifruit) hybrid population

Lena G Fraser1*, Alan G Seal2, Mirco Montefiori1, Tony K McGhie3, Gianna K Tsang1, Paul M Datson1, Elena Hilario1, Hinga E Marsh2, Juanita K Dunn2, Roger P Hellens1, Kevin M Davies3, Mark A McNeilage1, H Nihal De Silva1 and Andrew C Allan1

Author Affiliations

1 The New Zealand Institute for Plant & Food Research Limited, 120 Mt. Albert Road, Auckland, 1142, New Zealand

2 The New Zealand Institute for Plant & Food Research Limited, 412 No. 1 Road, RD 2, Te Puke, 3182, New Zealand

3 The New Zealand Institute for Plant & Food Research Limited, Fitzherbert Science Centre, Batchelar Road, Palmerston North, 4474, New Zealand

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BMC Genomics 2013, 14:28  doi:10.1186/1471-2164-14-28

Published: 16 January 2013

Additional files

Additional file 1:

Pedigree of the four Actinidia families that show segregation of petal colour. All the parents involved in creating the four F2 backcross families that demonstrated segregation of petal colour were from two Actinidia taxa, A. chinensis var. chinensis and A. eriantha. The parents of each generation are shown. The female parent is indicated by a pink blaze, and the male parent by blue. A. eriantha contributed the red petal phenotype to the segregating population.

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Additional file 2:

Alleles of MYB110a carried by the parents of the four F2 backcross Actinidia families. The alleles of MYB110a that are present in all parents of the backcross are represented by the sizes of the PCR products amplified by the primers of the marker Ke923. Where experimental data could not be generated, through loss of the parental genotype, the sizes of the alleles were inferred from the parents and progeny of that genotype.

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Additional file 3:

Red expression in petals, anther filaments and ovaries in the flowers of four F2 backcross hybrid Actinidia families. Ovary colour was independent of petal colour. Stamen filament colour was also independent of petal and ovary colour. Red filaments were found with both red or white petals, and red or green ovaries, in all combinations in female progeny, and with red or white petals in males.

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Additional file 4:

Anthocyanin and flavonoid analysis data. Identities and concentrations of anthocyanins and flavonols extracted from red petals of flowers of four inter-related F2 backcross Actinidia families.

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Additional file 5:

Gene-specific primer sequences. Quantitative real-time PCR was carried out with gene-specific primers that were designed using Vector NTI 9.0.0. PCR primers of marker Ke923 specific for MYB110a, and marker Ke701 specific for MYB110b were designed by LGF. (DOC 32 kb)

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