|The chromosome quotients of sequences tested for Y-linkage with PCR|
|Sequence name||Number of female alignments||Number of male alignments||Normalized chromosome quotient|
|An. stephensi Y sequence 1||0||211||0.000|
|An. stephensi Y sequence 2||1||305||0.003|
|An. stephensi Y sequence 3||4||148||0.023|
|An. stephensi Y sequence 4||10||335||0.026|
|An. stephensi Y sequence 5||2||61||0.029|
|An. gambiae Y sequence 1||13||101||0.129|
All of the sequences listed amplified a PCR product exclusively in male genomic DNA, and all have chromosome quotients less than 0.3. We made an exception for sYG2 as it only has 29 alignments from male data, and the cutoff was 30 (see Methods). Even at lower stringency, sYG2 still had no female alignments so we decided to include it. The chromosome quotients for gYG1 and gYG2 were calculated from the transcripts because of the long introns present in the genes.
Hall et al.
Hall et al. BMC Genomics 2013 14:273 doi:10.1186/1471-2164-14-273