Expression profile of six Anopheles Y genes. (A) The expression profile of the three An. stephensi Y genes sYG1, sYG2, and sYG3. (B) The expression profile of the three An. gambiae Y genes gYG1, gYG2, and gYG3. For both species, a ribosomal protein gene (RPS4) was used as positive control. RT-PCR was performed with primer-sets that demonstrated male-specific amplification in PCR on genomic DNA (Additional file 1: Table S1). The expression profile was performed on time points spanning from 0–2 hour embryos to adults. Mixed larvae refers to mixed L1 to L4 instars; h, hour; d, day.
Hall et al. BMC Genomics 2013 14:273 doi:10.1186/1471-2164-14-273