Open Access Research article

Six novel Y chromosome genes in Anopheles mosquitoes discovered by independently sequencing males and females

Andrew Brantley Hall1, Yumin Qi1, Vladimir Timoshevskiy2, Maria V Sharakhova2, Igor V Sharakhov2 and Zhijian Tu1*

Author Affiliations

1 Department of Biochemistry, Virginia Tech, Blacksburg, VA, USA

2 Department of Entomology, Virginia Tech, Blacksburg, VA, USA

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BMC Genomics 2013, 14:273  doi:10.1186/1471-2164-14-273

Published: 23 April 2013

Additional files

Additional file 1: Figure S1:

The calculation of chromosome quotients.

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Additional file 2: Figure S2:

Autosome, X, and Y sequences differ in the number of alignments from male and female sequence data.

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Additional file 3:

Sequences referenced in the text including: the six Y genes, intergenic Y sequences tested for male-specific amplification, An. stephensi Y sequences, An. gambiae Y sequences, the autosomal homolog of sYG1, and the revised An. stephensi scaffold linking the autosomal homolog of sYG3 to a long scaffold.

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Additional file 4: Table S1:

Analysis of the male alignment threshold.

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Additional file 5: Table S2:

Statistics from the fragmented genome assemblies.

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Additional file 6: Table S3:

The coverage of the male and female sequence data used in the study.

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Additional file 7: Table S4:

The number of reads and read length from the RNA-seq data used in the study.

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Additional file 8: Table S5:

Sequences of the primers used in the study.

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Additional file 9:

Alignment and parameters used to infer phylogeny.

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