Differentially expressed tags and corresponding genes in Ste and Fer.A, Differentially expressed tags in Ste and Fer. The “x” axis represents fold-change of differentially expressed unique tags in two libraries. The “y” axis represents the number of unique tags (log10). Differentially accumulating unique tags with a 5-fold difference between libraries are shown in the red region (97.66%). The blue (0.24%) and green (2.07%) regions represent unique tags that are up- and down-regulated for more than 5 folds in the Fer and Ste libraries, respectively. B, Comparision of gene expression levels between the two libraries. For comparing gene expression levels between the two libraries, each library was normalized to 1 million tags. Red dots represent transcripts more prevalent in Fer library, green dots show those present at a lower frequency in Fer and blue dots indicate transcripts that did not change significantly. The parameters “FDR <0.001” and “log2 Ratio ≥ 1” were used as the threshold to judge the significance of gene expression difference.
Yan et al. BMC Genomics 2013 14:26 doi:10.1186/1471-2164-14-26