Table 2

Scaffolding value of different paired-read library combinations
No. of libraries* Most efficient Scaffold N50 Least efficient Scaffold N50
1 15 kb 163,475 PE 37,694
2 5 kb + 25 kb 522,027 PE + 3 kb 46,699
2 5 kb + 20 kb 474,308 PE + 5 kb 141,403
2 8 kb + 25 kb 470,890 PE + 25 kb 142,007
3 5 kb + 20 kb + 25 kb 834,964 PE + 3 kb + 5 kb 158,525
3 5 kb + 15 kb + 25 kb 789,954 PE + 3 kb + 8 kb 171,253
3 8 kb + 20 kb + 25 kb 726,289 PE + 3 kb + 25 kb 198,696
7 ALL 1,287,609 N/A N/A

*All libraries were normalized to 8.5x physical genome coverage, limited by the amount of available data for the paired-end (PE) library.

van Heesch et al.

van Heesch et al. BMC Genomics 2013 14:257   doi:10.1186/1471-2164-14-257

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