Open Access Highly Accessed Methodology article

Massively-parallel sequencing of genes on a single chromosome: a comparison of solution hybrid selection and flow sorting

Jamie K Teer13*, Jennifer J Johnston1, Sarah L Anzick2, Marbin Pineda2, Gary Stone2, NISC Comparative Sequencing Program11, Paul S Meltzer2, James C Mullikin1 and Leslie G Biesecker1

Author Affiliations

1 National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA

2 National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

3 Current address: H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA

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BMC Genomics 2013, 14:253  doi:10.1186/1471-2164-14-253

Published: 15 April 2013

Additional files

Additional file 1: Figure S1:

Distributions of read depths across different regions of interest using Solution Hybrid Selection (SHS) or Flow Sort (FS). Although FS showed lower average coverage, the coverage distribution was much sharper. Figure S2. Overlap of MPG and Breakdancer/Pindel calls in the SHS-PE (A) and SHS-PE low (B) libraries.

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