Additional file 1.

RT-PCR of oligonucleotides corresponding to highly conserved region of dsx1 (A) and dsx2 (B). The amplified cDNAs were analyzed by agarose gel electrophoresis. Lane M: molecular weight marker. Lane 1 to 10: D. magna (female), D. magna (male), D. pulex (female), D. pulex (male), D. galeata (female), D. galeata (male), C. dubia (female), C. dubia (male), M. macrocopa (female), M. macrocopa (male).

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Toyota et al. BMC Genomics 2013 14:239   doi:10.1186/1471-2164-14-239