Experimental annotation of transcriptionally active regions (TARs) in C. dubliniensis. Genomic plots are visualized using the GeneScapes genome browser (downloadable at “http://code.google.com/p/genescapes webcite”). The first row shows the experimental annotations revealed by this study (black arrows filled with white arrows) in comparison to the in silico predicted gene models (grey block arrows) in row two. RNA-Seq data are illustrated as wiggle plots. The third (forward strand, black) and fourth row (reverse strand, light grey) represent mapped reads from FLX sequencing while those from Illumina sequencing are shown in the fifth (blastospore condition) and sixth row (hyphae condition) in dark grey. Alterations made to the in silico predicted gene models are indicated by red boxes. (A) Experimental validation of four in silico predicted genes including UTR regions. (B) Annotation of a coding novel TAR (nTAR) and (C) a non-coding nTAR. (D) Detection of a novel splice site in CD36_60800. Alteration of the CDS region with an elongated ORF in CD36_62560 (E) and a shortenend ORF in CD36_65370 (F) due to correction of start sites. (G) Antisense transcription in the 3′-end of CD36_17160. (H) Two predicted uORFs in the 5′-UTR of CD36_64010.
Grumaz et al. BMC Genomics 2013 14:212 doi:10.1186/1471-2164-14-212