Scheme representing the conditions and the reads obtained from Hydra vulgaris for Illumina and 454 RNAseq. A) Left panel: number of intact and regenerating Hydra mixed to extract mRNA used in 454 sequencing. Right panel: length distribution of 454 reads after adapter trimming. B) Left panel: percentages of mRNA taken from Hydra in intact and regenerative conditions, as deduced from their respective number of reads sequenced. Right panel: length distribution of Illumina reads after barcode removal and adapter trimming.
Wenger and Galliot BMC Genomics 2013 14:204 doi:10.1186/1471-2164-14-204