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Transcriptome-based discovery of pathways and genes related to resistance against Fusarium head blight in wheat landrace Wangshuibai

Jin Xiao1, Xiahong Jin1, Xinping Jia12, Haiyan Wang1, Aizhong Cao1, Weiping Zhao3, Haiyan Pei1, Zhaokun Xue1, Liqiang He1, Qiguang Chen1 and Xiue Wang1*

Author Affiliations

1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, China

2 Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

3 School of Biology and Food Engineering, Chuzhou University, Chuzhou, 239000, People’s Republic of China

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BMC Genomics 2013, 14:197  doi:10.1186/1471-2164-14-197

Published: 21 March 2013

Additional files

Additional file 1: Table S1:

Output statistics of sequencing. Sample was the equally-mixed RNA from spikes at three infection stages, 12, 24, 48 hai of Fg and the non-inoculated spikes. Table S2. The network of unigenes in KEGG database. 30,657 unigenes were assigned to 121 KEGG pathways and the top ten representative networks were listed. Table S3. Information of primer sequences used in this study. Table S4.In silico mapping of genes with undetected expression in NAUH117 compared with that in Wangshuibai. Table S5. Twelve unigenes were detected to be up-regulated in Wangshuibai while were not expressed in NAUH117 based on Digital Gene expression assay and 8 out of them were confirmed by qRT-PCR.

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Additional file 2: Figure S1:

Length distribution of unigenes. Figure S2. The length (A) and species (B) distribution of unigenes and the proportion of unigenes with matches in nr and Swiss port databases. Figure S3. Saturation analysis of DGE sequencing. When the amount of tags reached 2M or higher, the increase of number of detected genes almost ceased for each library (indicated by the arrow).

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