Additional file 1:.
Psi-check2 reporter vector containing native or mutant MSTN 3′-UTR. Vector contains Renilla luciferase reporter gene, hRluc, which is used to monitor changes in expression of 3′-UTR as the result of miRNA binding. The firefly luciferase reporter gene, hluc, is used to correct luciferase signal for transfection efficiency. The 1500-bp MSTN 3′-UTR sequence containing miR-27b consensus sequence (wilde type or mutated) was inserted into the vector at the 3′ end of the hRluc reporter gene. (TIFF 28 kb)
Format: TIFF Size: 28KB Download file
Miretti et al. BMC Genomics 2013 14:194 doi:10.1186/1471-2164-14-194