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Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype

Silvia Miretti, Eugenio Martignani, Paolo Accornero and Mario Baratta*

Author Affiliations

Department of Veterinary Science, University of Torino, via Leonardo da Vinci 44, Grugliasco, 10095, Italy

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BMC Genomics 2013, 14:194  doi:10.1186/1471-2164-14-194

Published: 19 March 2013

Additional files

Additional file 1::

Psi-check2 reporter vector containing native or mutant MSTN 3-UTR. Vector contains Renilla luciferase reporter gene, hRluc, which is used to monitor changes in expression of 3-UTR as the result of miRNA binding. The firefly luciferase reporter gene, hluc, is used to correct luciferase signal for transfection efficiency. The 1500-bp MSTN 3-UTR sequence containing miR-27b consensus sequence (wilde type or mutated) was inserted into the vector at the 3 end of the hRluc reporter gene. (TIFF 28 kb)

Format: TIFF Size: 28KB Download file

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