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Ancient orphan crop joins modern era: gene-based SNP discovery and mapping in lentil

Andrew G Sharpe1*, Larissa Ramsay1, Lacey-Anne Sanderson2, Michael J Fedoruk2, Wayne E Clarke3, Rong Li1, Sateesh Kagale1, Perumal Vijayan2, Albert Vandenberg2 and Kirstin E Bett2*

Author Affiliations

1 National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

2 Department of Plant Sciences, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK, S7N 5A8, Canada

3 Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Pl., Saskatoon, SK, S7N 0X2, Canada

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BMC Genomics 2013, 14:192  doi:10.1186/1471-2164-14-192

Published: 18 March 2013

Additional files

Additional file 1:

Bioinformatics workflow for lentil SNP discovery and selection for the Illumina genotyping platform.

Format: DOCX Size: 164KB Download file

Open Data

Additional file 2:

Contig and SNP data including lists of all contigs, all SNPs, gene homology with Medicago or soybean, SNPs selected for KASP and GoldenGate assays, and the results of these assays on a panel of 11 lines.

Format: XLSX Size: 10MB Download file

Open Data

Additional file 3:

Classification of the gene models according to Arabidopsis GO Slim categories.

Format: XLSX Size: 47KB Download file

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Additional file 4:

Ks plots representing the frequencies of substitutions per synonymous site for aligned othologous contigs between M. truncatula and L. culinaris (a) and between L. culinaris and L. ervoides (b).

Format: PPTX Size: 134KB Download file

Open Data