Table 1

List of putative oocyst/sporozoite-specific proteins (POSPs) identified in this study
Gene ID Protein name EC number Biological process/molecular function aFold change of oocyst vs tachyzoite mRNA levels Protein probability
day 0 day 4 day 10
energy
TGME49_026300 oxidoreductase, putative 1.3.1.34 fatty acid β-oxidation 260 40 103 6.23E-11
TGME49_034570 peroxisomal multifunctional enzyme type 2, putative 1.1.1.35 fatty acid β-oxidation 108 12 17 2.22E-16
TGME49_042390 enoyl-CoA hydratase/isomerase family protein, putative 4.2.1.17 fatty acid β-oxidation 21 16 5 1.35E-12
TGME49_047500 acyl-coenzyme A oxidase, putative 1.3.3.36 fatty acid β-oxidation 92 37 49 6.76E-12
TGME49_066270 2-methylbutyryl-CoA dehydrogenase, putative 1.3.99.- fatty acid β-oxidation 7 <2 <2 1.00E-30
TGME49_115480 acyl-CoA oxidase, putative 1.3.3.36 fatty acid β-oxidation 183 71 151 1.91E-10
TGME49_032580 acetyl-coenzyme A synthetase, putative 6.2.1.1 glycolysis/gluconeogenesis 43 5 12 1.00E-30
TGME49_089930 phosphoenolpyruvate carboxykinase, putative 4.1.1.49 glycolysis/gluconeogenesis 30 3 6 8.70E-07
TGME49_016090 tropomyosin 1 alpha chain, putative - regulation of actin mechanics 247 11 19 5.66E-06
TGME49_027050 pyruvate dehydrogenase kinase, putative 2.7.11.2 regulation of pyruvate dehydrogenase 3 4 3 2.16E-07
TGME49_071210 4-alpha-glucanotransferase, putative 2.4.1.25/3.2.1.33 starch and glucose metabolism 5 254 26 1.11E-15
TGME49_003110 citrate synthase, putative 2.3.3.1 TCA cycle 35 5 7 6.32E-09
metabolism
TGME49_003340 alkyl sulfatase, putative 3.5.2.6 - 8 <2 2 2.10E-05
TGME49_003580 NAD-binding domain-containing protein - - n.a. n.a. n.a. 4.73E-12
TGME49_038200 alpha/beta hydrolase fold domain containing protein - - 27 3 4 7.77E-15
TGME49_065860 nit protein 2, putative - - 5 <2 <2 2.74E-11
TGME49_069120 oxidoreductase, putative 1.1.1.100 - 183 61 125 4.44E-16
TGME49_069400 short chain dehydrogenase/reductase domain-containing protein 1.1.1.100 - 11 <2 <2 2.42E-07
TGME49_106450 short chain dehydrogenase family protein, putative 1.1.1.100 - <2 222 82 6.97E-05
TGME49_111780 alcohol dehydrogenase class III, putative 1.1.1.284 - 4 <2 <2 4.61E-06
TGME49_003500 alanine dehydrogenase, putative 1.4.1.1 amino acid metabolism 6 29 4 1.11E-15
TGME49_005420 aspartate-semialdehyde dehydrogenase, putative 1.2.1.11 amino acid metabolism 4 <2 <2 2.44E-08
TGME49_016640 homoserine kinase, putative 2.7.1.39 amino acid metabolism 74 28 26 4.03E-12
TGME49_059180 cystathionine beta-synthase, putative 4.2.1.22 amino acid metabolism n.a. n.a. n.a. 8.37E-13
TGME49_069110 ornithine aminotransferase, putative 2.6.1.13 amino acid metabolism 394 123 247 2.54E-12
TGME49_078910 O-acetylserine (thiol) lyase, putative 2.5.1.47 amino acid metabolism 20 3 5 2.39E-10
TGME49_112930 cystathionine beta-lyase, putative 4.4.1.1 amino acid metabolism 23 2 3 2.90E-10
TGME49_115260 alanine dehydrogenase, putative 1.4.1.1 amino acid metabolism 98 211 331 2.89E-14
TGME49_019230 acetoacetyl-CoA synthetase, putative 6.2.1.16 butanoate metabolism <2 <2 <2 4.97E-10
TGME49_031900 acyl-CoA dehydrogenase, putative 1.3.99.7 fatty acid metabolism 31 32 21 6.51E-11
TGME49_003570 acyltransferase, putative 2.3.1.15 glycerophospholipid metabolism 2 <2 <2 3.08E-13
TGME49_010260 glycerol-3-phosphate dehydrogenase, putative 1.1.1.8 glycerophospholipid metabolism 188 14 32 6.84E-13
TGME49_082230 sulfate adenylyltransferas-adenylylsulfate kinase, putative 2.7.7.4 purine metabolism 52 5 10 7.38E-13
TGME49_107030 purine nucleoside phosphorylase 2.4.2.3 pyrimidine salvage pathway 12 2 3 1.11E-16
TGME49_003720 vitamin K epoxide reductase complex subunit 1, putative 1.1.4.1 terpenoid-quinone biosynthesis 9 21 23 1.63E-08
TGME49_002140 methylglutaconyl-CoA hydratase, putative 4.2.1.18 valine, leucine and isoleucine degradation 5 2 2 1.02E-06
TGME49_005430 isovaleryl-CoA dehydrogenase, putative 1.3.99.10 valine, leucine and isoleucine degradation 8 13 6 2.49E-10
TGME49_015250 thiamin pyrophosphokinase, putative 2.7.6.2 vitamin biosynthesis <2 <2 <2 1.03E-09
cell rescue, defence and virulence
TGME49_005660 F5/8 type C domain-containing protein (CCp1A) - - 114 11 14 1.00E-30
TGME49_023480 thrombospondin type 1 domain-containing protein - - 24 <2 <2 6.35E-07
TGME49_056040 LCCL domain-containing protein (CCp5B) - - 343 24 32 1.11E-15
TGME49_058370 Rhoptry kinase family protein ROP28 - - <2 46 23 2.51E-12
TGME49_063410 scavenger receptor protein TgSR2 - - 144 12 12 2.22E-15
TGME49_064070 antigenic protein, putative (CCp1B) - - 76 7 8 1.10E-10
TGME49_067410 scavenger receptor protein TgSR1 - - 306 26 29 2.21E-08
TGME49_027150 glutaredoxin, putative - detoxification of oxygen radicals -3 -3 -3 6.84E-05
TGME49_116190 superoxide dismutase, putative 1.15.1.1 detoxification of oxygen radicals <2 589 470 3.76E-12
TGME49_009920 PAN domain-containing protein - host cell attachment/invasion <2 557 142 2.33E-14
TGME49_032400 PAN domain-containing protein - host cell attachment/invasion <2 44 8 7.77E-15
TGME49_038460 SRS22B - host cell attachment/invasion <2 <2 <2 8.95E-05
TGME49_058550 SRS28 (SporoSAG) - host cell attachment/invasion 2 323 312 1.44E-14
TGME49_058810 SRS27B - host cell attachment/invasion <2 153 46 4.84E-09
TGME49_065120 hypothetical protein, conserved (RON2 iso2) - moving junction formation <2 28 13 8.17E-08
TGME49_094400 hypothetical protein (RON2 iso 1) - moving junction formation n.a. n.a. n.a. 5.89E-06
TGME49_115730 apical membrane antigen, putative (AMA1 iso1) - moving junction formation <2 16 4 4.98E-12
cell cycle and DNA processing
TGME49_006400 DNA photolyase, putative 4.1.99.3 DNA repair 34 252 99 1.82E-10
protein fate
TGME49_016150 oligoendopeptidase F, putative - - 213 25 52 1.21E-11
TGME49_031060 subtilase family serine protease, putative 3.4.21.66 - <2 2 <2 3.95E-07
TGME49_034950 protein kinase (incomplete catalytic triad) - - <2 2 5 2.22E-15
TGME49_044620 NEK kinase 2.7.12.2 - 2 <2 <2 1.11E-10
TGME49_046800 acylamino-acid-releasing enzyme, putative 3.4.19.1 - <2 -2 <2 2.10E-10
TGME49_047240 ubiquitin carboxyl-terminal hydrolase isozyme L5, putative 3.4.19.12 deubiquitination pathway 3 -2 <2 7.70E-11
TGME49_066780 protein kinase (incomplete catalytic triad) - - 3 <2 <2 4.22E-14
TGME49_089240 DnaJ domain-containing protein 1.3.1.38 - <2 <2 <2 4.52E-11
TGME49_095640 endothelin-converting enzyme, putative 3.4.24.71 - 27 3 4 3.79E-12
TGME49_107640 CMGC kinase, CK2 family 2.7.12.2 - 140 19 27 2.15E-12
TGVEG_058750 protein kinase domain-containing protein, putative 2.7.11.11 - n.a. n.a. n.a. 8.47E-07
cellular transport, transport facilities and transport routes
TGME49_065230 electron transfer flavoprotein-ubiquinone oxidoreductase, putative 1.5.5.1 - 8 3 3 1.13E-12
TGME49_114330 ABC transporter, putative 3.6.3.44 - 31 13 12 9.50E-05
protein synthesis
TGME49_020300 ribosomal protein L15, putative - translation <2 <2 <2 9.08E-05
biogenesis of cellular components
TGME49_043720 peroxisomal biogenesis factor 11 domain-containing protein - peroxisome fission 15 22 20 1.25E-05
TGME49_086250 oocyst wall protein, putative (OWP6) - oocyst environmental resistance 44 7 12 6.83E-10
other
TGGT1_064840 hypothetical protein - - n.a. n.a. n.a. 7.85E-08
TGGT1_071710 hypothetical protein, conserved - - n.a. n.a. n.a. 5.11E-12
TGGT1_090830 hypothetical protein, conserved - - n.a. n.a. n.a. 2.89E-05
TGGT1_099390 hypothetical protein, conserved - - n.a. n.a. n.a. 7.99E-14
TGGT1_122010 hypothetical protein, conserved - - n.a. n.a. n.a. 6.77E-14
TGGT1_122320 hypothetical protein, conserved - - n.a. n.a. n.a. 4.09E-13
TGME49_002100 hypothetical protein - - 847 425 583 1.36E-07
TGME49_002110 hypothetical protein - - 1163 321 751 7.05E-11
TGME49_003230 hypothetical protein - - 11 42 27 3.05E-09
TGME49_003890 hypothetical protein - - 29 150 77 1.80E-09
TGME49_007250 hypothetical protein - - 155 15 28 7.55E-07
TGME49_009470 hypothetical protein - - 476 63 155 7.17E-10
TGME49_009810 hypothetical protein - - <2 30 11 2.86E-12
TGME49_012030 hypothetical protein - - <2 9 5 8.40E-11
TGME49_016970 hypothetical protein, conserved - - -5 2 <2 1.44E-14
TGME49_020280 SCP-like domain-containing protein - - n.a. n.a. n.a. 1.00E-30
TGME49_023430 hypothetical protein - - <2 66 6 1.23E-09
TGME49_023830 fasciclin domain-containing protein - - n.a. n.a. n.a. 1.50E-08
TGME49_023900 hypothetical protein - - 4 <2 <2 4.63E-05
TGME49_025120 rRNA processing protein, putative - - n.a. n.a. n.a. 1.00E-09
TGME49_026230 hypothetical protein - - <2 21 21 3.78E-08
TGME49_028240 hypothetical protein - - <2 <2 <2 2.11E-13
TGME49_030530 hypothetical protein - - 220 22 33 1.99E-10
TGME49_030540 hypothetical protein - - 53 6 10 4.33E-08
TGME49_031390 hypothetical protein - - 618 69 138 1.46E-09
TGME49_037080 hypothetical protein - - 1315 582 905 1.56E-10
TGME49_038160 hypothetical protein - - <2 252 78 1.03E-13
TGME49_038910 hypothetical protein - - n.a. n.a. n.a. 3.50E-05
TGME49_040610 hypothetical protein, conserved - - 65 5 3 3.57E-08
TGME49_044410 hypothetical protein - - n.a. n.a. n.a. 1.67E-07
TGME49_048170 hypothetical protein - - <2 4 2 3.33E-14
TGME49_048230 hypothetical protein - - <2 <2 <2 7.93E-05
TGME49_054760 hypothetical protein - - 153 126 80 1.12E-07
TGME49_055380 hypothetical protein - - 917 150 228 8.39E-12
TGME49_057690 hypothetical protein - - 16 3 3 2.10E-10
TGME49_058200 glutamic acid-rich protein, putative - - <2 48 31 6.43E-09
TGME49_058380 hypothetical protein - - <2 <2 <2 6.17E-05
TGME49_059670 von Willebrand factor type A domain-containing protein - - <2 257 41 2.22E-15
TGME49_062470 beta antigen, putative - - 658 129 203 5.09E-05
TGME49_065130 hypothetical protein - - <2 <2 <2 3.30E-11
TGME49_067980 hypothetical protein - - <2 9 5 2.01E-07
TGME49_068880 hypothetical protein - - -19 -3 -4 3.51E-09
TGME49_069350 hypothetical protein - - <2 <2 <2 3.29E-11
TGME49_070950 hypothetical protein - - 15 238 348 5.74E-08
TGME49_071490 hypothetical protein - - 198 148 282 6.91E-13
TGME49_073510 hypothetical protein - - <2 79 41 6.66E-15
TGME49_073670 hypothetical protein - - -2 <2 <2 3.79E-05
TGME49_073700 hypothetical protein - - 260 27 52 3.42E-08
TGME49_076860 late embryogenesis abundant domain-containing protein - - <2 556 390 2.22E-15
TGME49_076870 LEA1 protein, putative - - 8 497 146 2.03E-12
TGME49_078120 SCP-like extracellular domain-containing protein - - <2 96 66 5.81E-09
TGME49_078340 hypothetical protein - - 2 4 <2 6.67E-07
TGME49_079440 hypothetical protein, conserved - - 161 14 26 4.43E-09
TGME49_080430 hypothetical protein - - 1057 220 376 1.47E-07
TGME49_085690 notch (DSL) domain-containing protein - - 121 15 21 8.51E-12
TGME49_087840 hypothetical protein - - <2 <2 <2 3.00E-14
TGME49_088220 hypothetical protein - - 52 51 13 1.00E-30
TGME49_089740 hypothetical protein - - <2 -4 <2 3.44E-07
TGME49_090610 hypothetical protein - - 557 38 42 5.26E-08
TGME49_091840 hypothetical protein - - 260 15 26 1.00E-30
TGME49_093780 hypothetical protein - - -60 <2 <2 2.56E-06
TGME49_094600 hypothetical protein - - 15 506 557 3.19E-13
TGME49_097280 hypothetical protein, conserved - - <2 453 139 2.63E-13
TGME49_097410 hypothetical protein - - 187 31 72 1.38E-08
TGME49_106050 hypothetical protein - - 89 14 30 1.11E-15
TGME49_106500 hypothetical protein - - <2 <2 <2 6.28E-11
TGME49_109530 hypothetical protein - - <2 3 <2 1.96E-11
TGME49_109540 hypothetical protein - - <2 <2 <2 2.57E-10
TGME49_110160 piwi-PAZ domain-containing protein (TgAgo) - gene silencing 21 7 5 7.45E-12
TGME49_116890 hypothetical protein - - 46 5 9 5.18E-12
TGME49_119890 hypothetical protein - - -14 53 33 1.08E-09
TGME49_120280 hypothetical protein - - 3 16 12 7.52E-12
TGME49_120290 hypothetical protein - - 8 <2 <2 4.68E-11
TGME49_120500 patched transmembrane domain-containing protein - - 12 <2 <2 1.37E-07
TGME49_120710 hypothetical protein - - <2 9 4 2.15E-07
TGVEG_029770 hypothetical protein - - n.a. n.a. n.a. 6.87E-06
TGVEG_051660 hypothetical protein, conserved - - n.a. n.a. n.a. 8.83E-13
TGVEG_058740 hypothetical protein, conserved - - n.a. n.a. n.a. 2.22E-16
TGVEG_060360 hypothetical protein - - n.a. n.a. n.a. 1.28E-11
TGVEG_095590 hypothetical protein - - n.a. n.a. n.a. 3.27E-11

aFold change values refer to differences between the mRNA levels of unsporulated (day 0) or sporulated (day 4 and day 10) oocysts compared to day 2 in vitro tachyzoites of strain M4 [34].

Negative values indicate a downregulation in oocysts with respect to tachyzoites. Peak fold change values ≥2 are bolded. n.a., not available.

Possenti et al.

Possenti et al. BMC Genomics 2013 14:183   doi:10.1186/1471-2164-14-183

Open Data