Open Access Highly Accessed Methodology article

High-utility conserved avian microsatellite markers enable parentage and population studies across a wide range of species

Deborah A Dawson1*, Alexander D Ball14, Lewis G Spurgin12, David Martín-Gálvez15, Ian R K Stewart3, Gavin J Horsburgh1, Jonathan Potter1, Mercedes Molina-Morales16, Anthony W J Bicknell17, Stephanie A J Preston1, Robert Ekblom18, Jon Slate1 and Terry Burke1

Author Affiliations

1 Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK

2 School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK

3 Department of Biology, University of Delaware, Newark, DE, 19716, USA

4 Current address: Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK

5 Current address: Estación Experimental de Zonas Áridas (CSIC), Almería, E-04120, Spain

6 Current address: Departamento de Zoología, Universidad de Granada, Granada, E-18071, Spain

7 Current address: Plymouth University, Marine Biology and Ecology Research Centre, Davy Building, Drake Circus, Plymouth, PL4 8AA, UK

8 Current address: Department of Ecology and Genetics, Uppsala University, Norbyv. 18D, Uppsala, SE-75236, Sweden

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BMC Genomics 2013, 14:176  doi:10.1186/1471-2164-14-176

Published: 15 March 2013

Additional files

Additional file 1:

Genotypes observed for 12 species at 24 CAM loci.

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Additional file 2:

Full sequence data for 24 conserved avian microsatellite (CAM) loci.

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Additional file 3:

Primer melting temperatures in zebra finch and chicken for 24 conserved avian microsatellite (CAM) markers.

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Additional file 4:

Allelic richness versus genetic distance for each CAM marker.

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Additional file 5:

Typical numbers of loci polymorphic among those amplifying for a selection of passerine and non-passerine species from a selection of different studies.

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Additional file 6:

Homology of CAM loci to expressed sequence tags (ESTs), genes, other microsatellites and BACs.

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Additional file 7:

Estimated null allele frequencies at CAM loci for six species.

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