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Open Access Highly Accessed Methodology article

High-utility conserved avian microsatellite markers enable parentage and population studies across a wide range of species

Deborah A Dawson1*, Alexander D Ball14, Lewis G Spurgin12, David Martín-Gálvez15, Ian R K Stewart3, Gavin J Horsburgh1, Jonathan Potter1, Mercedes Molina-Morales16, Anthony W J Bicknell17, Stephanie A J Preston1, Robert Ekblom18, Jon Slate1 and Terry Burke1

Author affiliations

1 Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK

2 School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK

3 Department of Biology, University of Delaware, Newark, DE, 19716, USA

4 Current address: Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK

5 Current address: Estación Experimental de Zonas Áridas (CSIC), Almería, E-04120, Spain

6 Current address: Departamento de Zoología, Universidad de Granada, Granada, E-18071, Spain

7 Current address: Plymouth University, Marine Biology and Ecology Research Centre, Davy Building, Drake Circus, Plymouth, PL4 8AA, UK

8 Current address: Department of Ecology and Genetics, Uppsala University, Norbyv. 18D, Uppsala, SE-75236, Sweden

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Citation and License

BMC Genomics 2013, 14:176  doi:10.1186/1471-2164-14-176

Published: 15 March 2013

Abstract

Background

Microsatellites are widely used for many genetic studies. In contrast to single nucleotide polymorphism (SNP) and genotyping-by-sequencing methods, they are readily typed in samples of low DNA quality/concentration (e.g. museum/non-invasive samples), and enable the quick, cheap identification of species, hybrids, clones and ploidy. Microsatellites also have the highest cross-species utility of all types of markers used for genotyping, but, despite this, when isolated from a single species, only a relatively small proportion will be of utility. Marker development of any type requires skill and time. The availability of sufficient “off-the-shelf” markers that are suitable for genotyping a wide range of species would not only save resources but also uniquely enable new comparisons of diversity among taxa at the same set of loci. No other marker types are capable of enabling this. We therefore developed a set of avian microsatellite markers with enhanced cross-species utility.

Results

We selected highly-conserved sequences with a high number of repeat units in both of two genetically distant species. Twenty-four primer sets were designed from homologous sequences that possessed at least eight repeat units in both the zebra finch (Taeniopygia guttata) and chicken (Gallus gallus). Each primer sequence was a complete match to zebra finch and, after accounting for degenerate bases, at least 86% similar to chicken. We assessed primer-set utility by genotyping individuals belonging to eight passerine and four non-passerine species. The majority of the new Conserved Avian Microsatellite (CAM) markers amplified in all 12 species tested (on average, 94% in passerines and 95% in non-passerines). This new marker set is of especially high utility in passerines, with a mean 68% of loci polymorphic per species, compared with 42% in non-passerine species.

Conclusions

When combined with previously described conserved loci, this new set of conserved markers will not only reduce the necessity and expense of microsatellite isolation for a wide range of genetic studies, including avian parentage and population analyses, but will also now enable comparisons of genetic diversity among different species (and populations) at the same set of loci, with no or reduced bias. Finally, the approach used here can be applied to other taxa in which appropriate genome sequences are available.