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Transcriptional analysis of genes involved in nodulation in soybean roots inoculated with Bradyrhizobium japonicum strain CPAC 15

Gesiele Almeida Barros de Carvalho12, Jesiane Stefânia Silva Batista1, Francismar Corrêa Marcelino-Guimarães1, Leandro Costa do Nascimento3 and Mariangela Hungria1*

Author Affiliations

1 Empresa Brasileira de Pesquisa Agropecuária, CNPSo, PO Box 231, Londrina, Paraná 86001-970, Brazil

2 Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, PO Box 6001, Londrina, Paraná 86051-990, Brazil

3 Laboratório de Genômica e Expressão, Institute of Biology, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255, Campinas, São Paulo 13083-862, Brazil

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BMC Genomics 2013, 14:153  doi:10.1186/1471-2164-14-153

Published: 6 March 2013

Additional files

Additional file 1: Table S1:

Real- time PCR result of differential expression of two target genes from soybean roots inoculated (sample) and mock-inoculated (calibrator) using 2-∆∆Ct method. Table S2. Genes which encode enzymes present in the glycolytic pathway (A) and in the Krebs cycle (B) found in the subtractive library of soybean cv. Conquista when inoculated with B. japonicum strain CPAC 15. Table S3- Sequences of the primers used in the RT-qPCR and sizes of the PCR products obtained.

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Additional file 2: Figure S1:

Glycolysis pathway during nodulation of soybean cultivar Conquista at 10 days after inoculation with B. japonicum CPAC 15 (ID Kegg map00010). Figure S2. Krebs cycle of soybean cultivar Conquista at 10 days after inoculation with B. japonicum CPAC 15 (ID Kegg map00020). Figure S3. Glutathione metabolism – the antioxidant defense system present in the nodulation (ID Kegg- map00480). Figure S4. Carbohydrate metabolism involved in the reorganization of the plant cell wall during organogenesis of the nodule (ID Kegg-map00500). Figure S5. Spots of whole-cell protein extracts of soybean roots inoculated (left images) and non-inoculated (right images) with B. japonicum. Proteins identified corresponds to sucrose synthase (1) and putative glutathione-S-transferase (2).

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