Table 7

RT-PCR determination of gene expression in the choroid plexus
3 hr 1 day
NCBI gene symbol Control AMPH EIH AMPH EIH
Apold1(Verge)a 0.03 ± 0.002 0.06 ± 0.02 (2.00) 0.06 ± 0.07 (2.00) N.D. N.D.
Bmp4a,c 0.55 ± 0.04 3.65 ± 1.26 (6.64) 0.84 ± 0.23 (1.53) N.D. N.D.
Cebpda,b 0.01 ± 0.003 0.24 ± 0.09 (24.00) 0.15 ± 0.13 (15.00) N.D. N.D.
Cldn1 5.74 ± 0.70 5.73 ± 0.98 (1.00) 6.49 ± 0.80 (1.13) N.D. N.D.
Cxcl1a,b 0.004 ± 0.002 0.40 ± 0.62 (100.00) 0.30 ± 0.51 (75.00) N.D. N.D.
Cyr61a,b 0.24 ± 0.06 3.54 ± 2.70 (14.75) 3.16 ± 5.10 (13.17) N.D. N.D.
Dio2a 0.01 ± 0.005 4.34 ± 2.03 (434.00) 1.08 ± 1.13 (108.00) N.D. N.D.
Edn1b 0.003 ± 0.001 0.005 ± 0.002 (1.67) 0.011 ± 0.010 (3.67) 0.003 ± 0.004 (1.50) 0.002 ± 0.000
Fstb,d 0.03 ± 0.02 0.06 ± 0.02 (2.00) 0.10 ± 0.06 (3.33) 0.03 ± 0.02 (3.00) 0.01 ± 0.003
Has1a,b 0.09 ± 0.06 0.17 ± 0.03 (1.89) 0.17 ± 0.02 (1.89) 0.11 ± 0.02 (1.10) 0.10 ± 0.02
Lbpa 0.11 ± 0.06 0.53 ± 0.32 (4.81) 0.24 ± 0.12 (2.18) 0.97 ± 0.57 (2.11) 0.46 ± 0.31
Nat3b 0.01 ± 0.004 0.01 ± 0.004 (1.00) 0.01 ± 0.008 (1.00) 0.004 ± 0.003 (0.50) 0.008 ± 0.010
Nos3 0.06 ± 0.03 0.09 ± 0.04 (1.50) 0.10 ± 0.06 (1.67) 0.08 ± 0.05 (1.33) 0.06 ± 0.01
Oclna 4.96 ± 0.52 7.24 ± 1.12 (1.46) 6.01 ± 1.76 (1.21) N.D. N.D.
Reg3b N.E. N.E. N.E. N.E. N.E.
Sct N.E. N.E. N.E. N.E. N.E.
Sectm1ba 0.01 ± 0.002 0.46 ± 0.15 (46.00) 0.15 ± 0.14 (15.00) N.D. N.D.

All genes are expressed as a percentage of Gapdh, and are shown as the mean plus/minus standard error of the mean. Fold-changes of treatment to control are given in parentheses at the 3 hr time point. At the 1 day time point, fold-changes (in parentheses) are expressed as AMPH to EIH. The number of RNA aliquots (two to three animals per aliquot) used to determine expression per treatment group were as follows: 3 hr control, n = 7; 3 hr AMPH, n = 7; 3 hr EIH, n = 7; 1 day AMPH, n = 8; 1 day EIH, n = 7.

N.E. means gene not expressed (mean Ct value > 35).

N.D. designates expression not determined.

Symbols indicating statistically significant difference between treatments are found as a superscript after the gene name (first column).

a AMPH significantly differs from control at 3 hr (p < 0.05).

b EIH significantly differs from control at 3 hr (p < 0.05).

c AMPH significantly differs from EIH at 3 hr (p < 0.05).

d AMPH significantly differs from EIH at 1 day (p < 0.05).

Bowyer et al.

Bowyer et al. BMC Genomics 2013 14:147   doi:10.1186/1471-2164-14-147

Open Data