Table 5

RT-PCR determination of gene expression in MAV, striatum, and parietal cortex
3 hr 1 day
NCBI gene symbol Brain Region Control AMPH EIH AMPH EIH
Angpt2 MAV 1.08 ± 0.37 0.82 ± 0.23 (0.76) 0.81 ± 0.17 (0.75) N.D. N.D.
Parietal Cortex 0.02 ± 0.004 0.04 ± 0.01 (2.00) 0.03 ± 0.01 (1.50) N.D. N.D.
Striatum 0.03 ± 0.001 0.06 ± 0.02 (2.00) 0.04 ± 0.01 (1.33) N.D. N.D.
Aqp1 MAV 6.67 ± 1.61 5.30 ± 1.89 (0.79) 5.68 ± 1.11 (0.85) N.D. N.D.
Parietal Cortex 0.01 ± 0.003 0.08 ± 0.17 (8.00) 0.02 ± 0.02 (2.00) N.D. N.D.
Striatum 0.02 ± 0.003 0.04 ± 0.01 (2.00) 0.02 ± 0.003 (1.00) N.D. N.D.
Aqp4 MAVb,c 1.00 ± 0.34 1.11 ± 0.27 (1.11) 1.74 ± 0.11 (1.74) N.D. N.D.
Parietal Cortex 0.75 ± 0.03 0.73 ± 0.12 (0.97) 0.79 ± 0.10 (1.05) N.D. N.D.
Striatum 1.86 ± 0.01 2.14 ± 0.24 (1.15) 2.01 ± 0.36 (1.08) N.D. N.D.
Cldn5 MAVa 3.95 ± 1.18 0.99 ± 0.52 (0.25) 1.53 ± 0.88 (0.39) N.D. N.D.
Parietal Cortex 0.41 ± 0.06 0.19 ± 0.05 (0.46) 0.19 ± 0.12 (0.46) N.D. N.D.
Striatum 0.45 ± 0.04 0.20 ± 0.06 (0.44) 0.28 ± 0.13 (0.62) N.D. N.D.
Esam MAV 3.55 ± 1.16 4.29 ± 1.19 (1.21) 2.68 ± 0.42 (0.75) N.D. N.D.
Parietal Cortex 0.25 ± 0.03 0.66 ± 0.14 (2.64) 0.68 ± 0.42 (2.72) N.D. N.D.
Striatum 0.35 ± 0.02 0.80 ± 0.21 (2.29) 0.55 ± 0.04 (1.57) N.D. N.D.
F11r MAVa,b 1.63 ± 0.27 0.96 ± 0.15 (0.59) 0.96 ± 0.20 (0.59) N.D. N.D.
Parietal Cortex 0.11 ± 0.02 0.10 ± 0.01 (0.91) 0.10 ± 0.03 (0.91) N.D. N.D.
Striatum 0.19 ± 0.02 0.15 ± 0.01 (0.79) 0.16 ± 0.05 (0.84) N.D. N.D.
Fst MAVa,d 0.11 ± 0.05 2.23 ± 1.33 (20.27) 0.33 ± 0.15 (3.00) 0.25 ± 0.16 (2.78) 0.09 ± 0.02
Parietal Cortex 0.11 ± 0.05 0.21 ± 0.06 (1.91) 0.22 ± 0.04 (2.00) N.D. N.D.
Striatum 0.19 ± 0.05 0.40 ± 0.20 (2.10) 0.33 ± 0.04 (1.74) N.D. N.D.
Has1 MAVa,d 0.05 ± 0.02 0.20 ± 0.08 (2.0) 0.760 ± 0.670 (15.20) 0.21 ± 0.07 (1.75) 0.12 ± 0.02
Parietal Cortex 0.04 ± 0.01 0.11 ± 0.03 (2.75) 0.13 ± 0.04 (3.25) N.D. N.D.
Striatum 0.03 ± 0.01 0.07 ± 0.03 (2.33) 0.06 ± 0.01 (2.00) N.D. N.D.
Lbp MAVd 2.22 ± 0.86 3.52 ± 2.43 (1.58) 1.40 ± 0.48 (0.63) 12.05 ± 7.42 (5.06) 2.38 ± 1.12
Reg3a MAVa,d 0.001 ± 0.001 0.45 ± 0.50 (450.00) 0.01 ± 0.01 (10.00) N.D. N.D.
Parietal Cortex 0.001 ± 0.001 0.002 ± 0.001 (2.00) 0.005 ± 0.00 (5.00) N.D. N.D.
Striatum 0.01 ± 0.01 0.02 ± 0.01 (2.00) 0.03 ± 0.004 (3.00) N.D. N.D.
Reg3b MAVa,d 0.001 ± 0.001 0.21 ± 0.27 (210.00) 0.01 ± 0.004 (10.00) 0.44 ± 0.36 (62.86) 0.007 ± 0.014
Parietal Cortex 0.001 ± 0.00 0.001 ± 0.00 (1.00) 0.001 ± 0.00 (1.00) N.D. N.D.
Striatum 0.0005 ± 0.0001 0.0007 ± 0.0002 (1.40) 0.0005 ± 0.0003 (1.00) N.D. N.D.
Reg3g MAVa 0.003 ± 0.002 0.31 ± 0.33 (103.33) 0.02 ± 0.01 (6.67) 0.19 ± 0.26 (47.50) 0.004 ± 0.005
Sct MAVa,d 0.004 ± 0.001 0.025 ± 0.017 (6.25) 0.006 ± 0.002 (1.50) 0.014 ± 0.004 (3.50) 0.004 ± 0.002
Slc15a1 MAVa,c 0.19 ± 0.09 3.19 ± 5.72 (16.79) 0.16 ± 0.07 (0.84) 0.04 ± 0.04 (1.33) 0.03 ± 0.02
Parietal Cortex 0.03 ± 0.003 0.04 ± 0.01 (1.33) 0.03 ± 0.003 (1.00) N.D. N.D.
Striatum 0.04 ± 0.01 0.05 ± 0.01 (1.25) 0.04 ± 0.01 (1.00) N.D. N.D.
Sox18 MAVa 5.57 ± 2.58 0.96 ± 0.33 (0.17) 2.11 ± 1.54 (0.38) N.D. N.D.
Parietal Cortex 0.36 ± 0.06 0.17 ± 0.07 (0.47) 0.24 ± 0.20 (0.67) N.D. N.D.
Striatum 0.46 ± 0.10 0.23 ± 0.08 (0.50) 0.26 ± 0.25 (0.56) N.D. N.D.

All genes are expressed as a percentage of Gapdh and are shown as the mean plus/minus standard error of the mean. Fold-changes of treatment to control are given in parentheses at the 3 hr time point. At the 1 day time point, fold-changes (in parentheses) are expressed as AMPH to EIH. All the RT-PCR primers appeared to function optimally except those for Slc15a1 which gave a somewhat bimodal melt curve (several sets of primers were tried). The number of RNA aliquots (one per animal) used to determine expression per treatment group were as follows: 3 hr control, n = 6; 3 hr AMPH, n = 7; 3 hr EIH, n = 6; 1 day AMPH, n = 6; 1 day EIH, n = 6.

N.D. designates expression not determined.

Symbols indicating statistically significant difference between treatments are found as a superscript after the brain region (second column).

a AMPH significantly differs from control at 3 hr (p < 0.05).

b EIH significantly differs from control at 3 hr (p < 0.05).

c AMPH significantly differs from EIH at 3 hr (p < 0.05).

d AMPH significantly differs from EIH at 1 day (p < 0.05).

Bowyer et al.

Bowyer et al. BMC Genomics 2013 14:147   doi:10.1186/1471-2164-14-147

Open Data