Additional file 5: Figure S4.
Whole genome expression after modulation of miR-30a expression. MCF7 cells were transiently transfected with different conditions, miR-30a-k.d., Pre-miR-30a, and control miR-159-k.d. After 48 hours, RNA was extracted from biological triplicates, and interrogated for whole genome expression with Illumina HT12 bead arrays. A. Scatter plot between 2 technical replicates indicating a proper correlation. B. Cluster analysis and heat-map including all probes differentially expressed between Pre-miR-30a and miR-159 control k.d. As can be seen, no transcripts were significantly different between miR-30a-k.d. and miR-159 control k.d. C. Expression of selected miRNAs was validated by qRT-PCR in independent biological triplicates. Downregulation was as expected in cells overexpressing miR-30a (Pre-miR-30a), while no differences were found after miR-30a k.d, confirming the results from the whole genome expression assay. P values under 0.05 (two-tailed student t test) are represented with an asterisk (*).
Format: TIFF Size: 5.4MB Download file
Ouzounova et al. BMC Genomics 2013 14:139 doi:10.1186/1471-2164-14-139