Table 1

Differentially expressed (>2 log2) genes in HepG2 cells after treatment with Padma 28
Microarray (n=1) qPCR (n=5)
Gene Gene title log2 (ratio) Fold Fold P(H1)
CYP1A1 cytochrome P450, family 1, subfamily A1 8.7 408.4 123.4 ± 31.1 0.001
PTGR1 prostaglandin reductase 1 4.8 27.4
SLC7A11 solute carrier family 7, member 11 4.2 18.9
AKR1B10 aldo-keto reductase family 1, member B10 3.9 14.7 13.9 ± 4.9 0.003
ASNS asparagine synthetase 3.4 10.2 18.8 ± 5.5 0.008
CYP24A1 cytochrome P450, family 24, subfamily A 1 3.1 8.4 5.0 ± 0.9 0.132
PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1 3.0 8.2
NUPR1 nuclear protein 1 2.6 6.3
SCHIP1 schwannomin interacting protein 1 2.6 6.2
GSTA1* glutathione S-transferase alpha 1 2.6 6.1 4.5 ± 1.3 0.036
FECH ferrochelatase 2.4 5.2 3.7 ± 0.8 0.018
CD14 CD14 molecule 2.4 5.2
AKR1C2** aldo-keto reductase family 1, member C2 2.4 5.1 6.7 ± 1.2 0.007
LOC374443 CLR pseudogene 2.3 5.0
BCAT1 branched chain aminotransferase 1, cytosolic 2.3 5.0
KLHDC9 kelch domain containing 9 2.2 4.6
HELLS helicase, lymphoid-specific 2.2 4.4
CARS cysteinyl-tRNA synthetase 2.1 4.3 3.2 ± 0.6 0.001
AKR1C1** aldo-keto reductase family 1, member C1 2.1 4.2 6.7 ± 1.2 0.007
ABCC4 ATP-binding cassette, sub-family C, member 4 2.1 4.2
PIR pirin (iron-binding nuclear protein) 2.1 4.2
RAB27A RAB27A, member RAS oncogene family 2.1 4.2
HMOX1 haeme oxygenase (decycling) 1 2.1 4.2 5.3 ± 0.8 0.008
GCLM glutamate-cysteine ligase, modifier subunit 2.1 4.1
RBM39 RNA binding motif protein 39 -2.8 -7.2 1.1 ± 0.6 0.083
DKK1 dickkopf homolog 1 (Xenopus laevis) -2.7 -6.7 -24.2 ± 5.9 0.005
SCN1A sodium channel, voltage-gated, type I, alpha subunit -2.6 -6.0 -30.3 ± 10 0.002
SLC13A3 solute carrier family 13, member A3 -2.4 -5.2
MT1M metallothionein 1M -2.3 -4.9
LGR5 leucine-rich G protein-coupled receptor 5 -2.1 -4.4 -8.1 ± 1.9 0.001
SLC26A3 solute carrier family 26, member 3 -2.1 -4.2
SH3PXD2A SH3 and PX domains 2A -2.0 -4.1
SUCLG1 succinate-CoA ligase, alpha subunit -2.0 -4.1 0.3 ± 0.6 0.786

*primers detect GSTA1-A3 and A5; **primers detect AKR1C1-C4a.

The P(H1) value calculated for the qPCR experiments indicates the probability that the difference between sample and control group is significant.

Klein et al.

Klein et al. BMC Genomics 2013 14:133   doi:10.1186/1471-2164-14-133

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