Workflow of the method for the analysis of gene expression and copy number changes. The figure illustrates the strategy to achieve a combined paired analysis of data sets from genome-wide microarrays both for GE and CN. The method searches for high correlation between segmented chromosomal regions that present a significant CN alteration (gain or loss) and segmented regions with significant GE change (up or down). The frequency of alteration is also analysed. The selected loci (hotspots) are regions chosen as significantly correlated and frequently altered in both GE and CN.
Fontanillo et al. BMC Genomics 2012 13(Suppl 5):S5 doi:10.1186/1471-2164-13-S5-S5