Mutations predicted to affect structure often impact function. We investigated the subset of mutants predicted by our method to change structure from uncertain predictions (>0.5) to very strong predictions (>0.9). Residues in this subset, we found more often than expected to also have an observed effect in other data sets than used for our method, namely on protein stability (solid line) and protein function (dashed line). This suggests that strong structural change upon amino acid change results in increased likelihood to alter function or stability.
Schaefer and Rost BMC Genomics 2012 13(Suppl 4):S4 doi:10.1186/1471-2164-13-S4-S4