Table 1

Summary of analysis on miRNA reads

G1245N

G1245T


# Reads

17,492,248

23,974,839

# detected pre-miRNAsa

542

556

# detected miRNAsa

688

705

# miRNAs at opposite armb

87

96

# isomiRs

5,132

5,305

# isomiRs with length equal to miRNA

542

569


We mapped the sequence reads back to human pre-miRNA, quantifying the expression levels of pre-miRNAs, mature miRNA and isomiRs.

a: The human pre-miRNA and mature miRNA are based on the definition of miRBase 16. Totally, there are 1,048 pre-miRNAs and 1,223 mature miRNAs, resulting in 1,366 miRNA/pre-miRNA pairs.

b: According to miRBase 16, 730 out of 1,048 human pre-miRNAs encode only one mature miRNA at one arm, either 5p or 3p arm. With the improvement on NGS's sequencing intensity, we detected additional opposite-arm mature miRNAs at the pre-miRNAs originally encoding only one miRNA.

Li et al. BMC Genomics 2012 13(Suppl 1):S13   doi:10.1186/1471-2164-13-S1-S13

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