Additional file 4.

The difficulty in distinguishing nucleotide addition from nucleotide modification. In this case, the last nucleotide variation could be generated from nucleotide modification from C to U at the terminus of the sequence read with 22 nucleotides, which does not alter the length of the read. However, it could also be generated from nucleotide addition of U to the terminus of the read with 21 nucleotides, which lengthens the read by one nucleotide.

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Li et al. BMC Genomics 2012 13(Suppl 1):S13   doi:10.1186/1471-2164-13-S1-S13