Detection of 5p arm miRNA of hsa-mir-1307. According to miRBase annotation, hsa-mir-1307 encodes mature miRNA at only its 3p arm. We used stem-loop RT-PCR to succeed in detecting the additional opposite-arm miRNA in both G1245N and G1245T tissue.
Li et al. BMC Genomics 2012 13(Suppl 1):S13 doi:10.1186/1471-2164-13-S1-S13