Open Access Highly Accessed Research article

Large-scale functional RNAi screen in C. elegans identifies genes that regulate the dysfunction of mutant polyglutamine neurons

François-Xavier Lejeune1, Lilia Mesrob1, Frédéric Parmentier1, Cedric Bicep12, Rafael P Vazquez-Manrique12, J Alex Parker123, Jean-Philippe Vert456, Cendrine Tourette127 and Christian Neri12*

Author Affiliations

1 INSERM, Unit 894, Laboratory of Neuronal Cell Biology and Pathology, 75014 Paris, France

2 University of Paris Descartes, EA 4059, 75014 Paris, France

3 Université de Montreal, CRCHUM Centre d'excellence en neuromique, Hôpital Notre-Dame, Montreal, QC,H2W 1T8 Canada

4 Mines ParisTech, CBIO, Fontainebleau, 75006 Paris, France

5 Curie Institute, Research Center, 75005 Paris, France

6 INSERM, Unit 900, Paris, 75005 France

7 Buck Institute, Novato, CA 94945, USA

For all author emails, please log on.

BMC Genomics 2012, 13:91  doi:10.1186/1471-2164-13-91

Published: 13 March 2012

Additional files

Additional file 1:

Figure S1. Distribution of S scores for the primary screen in 128Q;rrf-3 nematodes. The graph shows the S scores for 4017 RNAi clones that did not elicit lethality or developmental abnormalities. The S score was calculated as [(Percent response - mean baseline)/mean baseline]. The maximally-achievable score is 4.55 (100% response to touch) and the smallest score is -1 (complete loss of touch response). The blue line indicate the mean baseline (S = 0). Dooted blue lines show the mean baseline ± SD*2.6. Clones with S scores falling outside the mean baseline ± SD*2.6 interval were retained for the secondary screen.

Format: JPEG Size: 200KB Download file

Open Data

Additional file 2:

Table S1. List of the 2211 genes that caused lethality and developmental abnormalities when knocked-down by RNAi.

Format: DOC Size: 1.6MB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Table S2. List of the 3823 genes that either showed no effect or modified 128Q-neuron dysfunction when knocked-down by RNAi in the primary screen.

Format: DOC Size: 3MB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Table S3. List of the 662 genes that modified 128Q-neuron dysfunction when knocked-down by RNAi in the secondary screen.

Format: DOC Size: 676KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 5:

Table S4. List of the 15 genes that modified 19Q-neuron dysfunction when knocked-down by RNAi.

Format: DOC Size: 43KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 6:

Table S5. List of the 15 genes that modified 128Q-neuron dysfunction in the secondary screen and were previously reported to modify polyQ aggregation when knocked-down by RNAi.

Format: DOC Size: 49KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 7:

Table S6. Gene Ontology classification of genes that suppressed 128Q-neuron dysfunction when knocked-down by RNAi.

Format: DOC Size: 839KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 8:

Table S7. Gene Ontology classification of genes that aggravated 128Q-neuron dysfunction when knocked-down by RNAi.

Format: DOC Size: 626KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 9:

Table S8. Modules (n = 137) generated by network-boosted analysis for suppression of 128Q-neuron dysfunction by RNAi.

Format: DOC Size: 233KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 10:

Table S9. Modules (n = 105) generated by network-boosted analysis for aggravation of 128Q-neuron dysfunction by RNAi.

Format: DOC Size: 183KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data