Additional File 5.

Supporting Figure S5: Simulations show that recovery of assigned amino acid-associated weights is positively correlated to antibody diversity. This Figure is complementary to Figure 3. Antibody binding profiles were simulated for antibody mixtures of 1 to 16348 different antibodies. The correlation (r) of simulated AAWS (<a onClick="popup('http://www.biomedcentral.com/1471-2164/13/79/mathml/M20','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2164/13/79/mathml/M20">View MathML</a>) with assigned AAWS (<a onClick="popup('http://www.biomedcentral.com/1471-2164/13/79/mathml/M8','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2164/13/79/mathml/M8">View MathML</a>) increases with increasing antibody diversity. Both a simulated random peptide library (Xsim) of 255 14-mers as well as assigned AAWS <a onClick="popup('http://www.biomedcentral.com/1471-2164/13/79/mathml/M8','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2164/13/79/mathml/M8">View MathML</a> were generated once and kept constant across the whole simulation. Simulated antibody binding profiles were computed with Equation 2, detailed in the results section. Corresponding AAWS were determined with Equation 1. For every mixture of nAb-different antibodies, 100 simulations with newly randomly generated antibody mixtures were run.

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Greiff et al. BMC Genomics 2012 13:79   doi:10.1186/1471-2164-13-79