Additional File 2.

Supporting Figure S2: Removing the signal of the secondary antibody accentuates differences between binding profiles of monoclonal and serum antibodies. (A) The predictive performance values (Q2) were calculated for monoclonal (mAb) as well as serum IgM (sIgM) and IgG (sIgG) antibody binding profiles before (blue) and after (red) correction of the measured log-transformed signal intensities by removal of the polyclonal secondary antibody-correlated signals using PLS. (B) Shown is the pairwise correlation (r) of the corresponding AAWS <a onClick="popup('','MathML',630,470);return false;" target="_blank" href="">View MathML</a>. For the two statistical measures, signal correction entails a significant decrease in the mAb median, whereas sIgM and sIgG medians remain largely unchanged. Both before and after secondary antibody correction of antibody binding profiles, sIgM profiles have higher predictive performance (Q2) and a higher median pairwise correlation (r) of AAWS than sIgG profiles. In (A) and (B), mAb signifies antibody binding profiles from 13 monoclonal antibodies and sIgM/sIgG serum IgM and serum IgG binding profiles from 58 BALB/c mice sera, respectively. Antibody binding profiles were measured with the standard peptide library of 255 14-mers. Corresponding AAWS (<a onClick="popup('','MathML',630,470);return false;" target="_blank" href="">View MathML</a>) were determined with Equation 1.

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Greiff et al. BMC Genomics 2012 13:79   doi:10.1186/1471-2164-13-79