Additional File 1.
Supporting Figure S1: Experimental setup: infection of BALB/c mice with Heligmosomoides bakeri and collection of blood samples at three different stages of immune response. Serum samples from 15 BALB/c mice raised under specific pathogen-free conditions were collected. These mice were infected with the intestinal nematode Heligmosomoides bakeri formerly known as Heligmosomoides polygyrus . Further serum samples were collected at 10 dpi (days post infection; 15 samples), at 14 dpi (13 samples), and at 18 dpi (15 samples) totaling 58 serum samples. The serum was isolated and subsequently incubated with random peptide libraries. We categorized the serum samples into healthy (0 dpi; 15 samples), acute phase (10 and 14 dpi; 15 and 13 samples respectively) and early chronic phase (18 dpi; 15 samples), thus delineating the three major stages of immune response of a mouse, before and after primary infection with HB. Practical experimental difficulties reduced the intended number of usable 14 dpi samples from 15 to 13.
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Greiff et al. BMC Genomics 2012 13:79 doi:10.1186/1471-2164-13-79