Table 2

Validation of the SNP scoring scheme by PCR-based re-sequencing
SNP score range Total
0–0.4 0.4–0.7 0.7–1.0 (0–1)
V NV V NV V NV V NV
No. of SNPs 9 63 25 8 996 35 1030 106
Percent validation 12.5 75.76 96.61 90.67

Fragments from selected loci were amplified by PCR and directly sequenced, and SNPs were identified using PolyPhred after base-calling. The table shows the number of SNPs identified in silico that have been validated (V) or not validated (NV), grouped by their score.

Ackermann et al.

Ackermann et al. BMC Genomics 2012 13:736   doi:10.1186/1471-2164-13-736

Open Data