Chromosome region view of three novel genes detected by proteogenomics. Loci of targets SPO_PG032 (Panel A) and targets SPO_PG027 and SPO_PG009 (Panel B) are represented. The six reading frames are shown with all coded stop codons represented by black dashes. Coloured in red are the nucleic sequences specifying the peptides detected by MS/MS. Panel A: The green line represents the area of the stop-to-stop sequence that shows homology to other proteins by means of a PSI-BLAST. The amino acid sequence in bold black represents the plausible sequence of SPO_PG032. Highlighted in red squares are the peptides detected by MS/MS. Panel B: Blue lines represent RT-PCR amplification attempts. Green rectangles show identified transcriptional terminators.
Christie-Oleza et al. BMC Genomics 2012 13:73 doi:10.1186/1471-2164-13-73