RT-PCR amplification for 'one-hit wonder' validation. A schematic view of the genomic region of target SPO_PG034 is shown as an example. The pink square represents the putative protein sequence highlighting in red the unique peptide detected by MS/MS. SPO0877 is shown with its conserved BLAST region (broken green line) and plausible non-coding area (grey crossed). In yellow is the mRNA produced from SPO_PG034 which was amplified with by RT-PCR using specific primers. The 3% agarose gel stained with ethidium bromide shows the five "one-hit wonder" targets from which RT-PCR amplification was obtained (lane "+"). Lanes marked with "-" represent negative controls by testing PCR amplification on RNA extractions to ensure total DNA elimination.
Christie-Oleza et al. BMC Genomics 2012 13:73 doi:10.1186/1471-2164-13-73